FastQCFastQC Report
Sat 18 Jun 2016
SRR3137962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4493214
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG199230.4434019835244883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36850.039.3501361
GTATCAA75250.032.3604471
ATCAACG89700.026.6547813
CAACGCA90900.026.3890025
TCAACGC94050.025.4267654
TATCAAC95250.025.1683982
AACGCAG98150.024.8630546
GCTCGAT6450.023.54510564
CTCGATA6600.022.48778765
CGTACGA4300.022.4721160
CGATAAT7100.021.87642367
ACGCAGA110750.021.4478557
CGCAGAG113450.020.8174928
TGGTCGT8050.019.72130651
AGAGTAC116450.019.42668511
GTACATG117150.019.4045371
GAGTACT67150.019.3101612
TCGTCTA8100.019.17434754
ACCGTCG5250.019.05414223
TACATGG117050.018.8918692
Produced by FastQC (version 0.11.5)