FastQCFastQC Report
Sat 18 Jun 2016
SRR3137962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4493214
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37650.040.521431
GTATCAA79450.033.2349471
ATCAACG90100.028.374773
CAACGCA91950.027.766055
TCAACGC94900.027.0119954
TATCAAC98750.026.2738952
AACGCAG99200.025.9098076
TATGCCG16450.024.95719743
TCTCGTA14850.024.16137538
CTCGTAT14700.023.93853639
GCTCGAT5850.023.58950664
AATCTCG15550.023.29558836
ACGCAGA112700.022.4684147
TCGTATG19000.022.33398840
CGCAGAG114600.021.8550728
ATGCCGT19150.021.25827444
TGCCGTC20350.020.85237145
TGCGACG5300.020.82951522
CGCACTA5300.020.17926858
ATCTCGT18150.020.1485637
Produced by FastQC (version 0.11.5)