FastQCFastQC Report
Sat 18 Jun 2016
SRR3137961_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137961_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684837
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG270290.7335195559532213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.037.0788651
GTATCAA75600.032.979031
CAATGCG6850.029.70883419
CGACGAG7150.029.42833324
GCTCGAT8200.027.78340164
ATCAACG90700.026.5838663
CAACGCA92200.026.4233065
CTCGATA8500.026.39706865
TATCAAC96350.025.485572
AACGCAG100600.024.7015696
TGCGACG8500.024.34794822
TCAACGC100850.024.0827224
GTACTAG12600.023.504391
ATGCGAC9400.022.75034521
TCGTCTA10150.022.10381154
ATCGTCA9300.021.8730810
GCTCGTC10100.021.8679239
TGGTCGT9850.021.72607451
ACGCAGA109450.021.669197
GTACATG115450.021.5956251
Produced by FastQC (version 0.11.5)