FastQCFastQC Report
Sat 18 Jun 2016
SRR3137961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684837
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG51760.14046754306906928RNA PCR Primer, Index 17 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37500.038.749491
GTATCAA77850.035.2027851
TATGCCG22900.031.3367843
CTCGATA7850.031.20435565
CTCGTAT19350.029.775339
TCGTATG24700.029.1924140
GCTCGAT8400.028.75049464
AATCTCG20250.028.45195236
CAACGCA94850.027.7881075
ATCTCGT21050.027.53453837
ATCAACG95900.027.4482573
ATGCCGT26150.026.9144244
TATCAAC101250.026.782682
TCTCGTA21900.026.62338338
TGGTCGT9150.026.39389451
CGATAAT9700.025.60868667
CGACGAG7300.025.51994524
TGCCGTC26650.025.50359745
AACGCAG105200.025.4477236
TCAACGC104100.025.4183644
Produced by FastQC (version 0.11.5)