FastQCFastQC Report
Sat 18 Jun 2016
SRR3137960_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137960_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264461
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG152080.4658655747457237No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG44960.13772564597953535No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC33360.1021914490631072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38650.039.0288351
GTATCAA78600.034.5712781
GCTCGAT10500.033.8601864
CGACGAG8950.031.99013124
CTCGATA11550.031.08178765
CAATGCG8950.030.82912419
TGGTCGT12100.030.51777651
ATCGTCA10450.028.37546510
TGCGACG10300.027.12660422
CAACGCA100300.026.8699385
ATCAACG98500.026.8233723
CGATAAT14100.026.68632367
GCTCGTC14050.026.52865839
TATCAAC104850.025.7839152
CTGGTCG14750.025.73644350
GTCGTCT14600.025.29213353
GTCTACC14800.025.18391456
AACGCAG109050.025.1610186
GTACTAG15200.025.1501351
TCGTCTA14850.025.0991254
Produced by FastQC (version 0.11.5)