FastQCFastQC Report
Sat 18 Jun 2016
SRR3137960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264461
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG53320.16333477410206465No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC36500.11181018857324379No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG35020.10727651517356158TruSeq Adapter, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37400.037.467871
CGACGAG8550.035.5070724
GTATCAA74550.034.1675031
CAATGCG9050.033.9255119
GCTCGAT11750.033.47278664
TATGCCG14950.033.2312143
TCGTATG15650.031.96528240
TGGTCGT12450.031.59126751
CTCGTAT14650.031.55673439
TGCGACG9850.030.47062122
CTCGATA13400.029.3511465
TCTCGTA15550.029.064738
ATCTCGT15450.029.02951237
GATCTCG15450.029.02951236
TCAGCGC14300.028.2276544
ATCAACG89450.027.8883463
CGATAAT14150.027.79499667
CGTACTA18200.027.48578629
CAACGCA91550.027.2859065
GCTCGTC14600.027.17501839
Produced by FastQC (version 0.11.5)