FastQCFastQC Report
Sat 18 Jun 2016
SRR3137959_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137959_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500915
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG118610.33879714303260716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG7550.037.00731324
GGTATCA34350.036.7900351
GTATCAA72600.035.1457251
CAATGCG8450.032.65327519
TGCGACG8950.031.21756422
ATCAACG87750.028.5766873
CAACGCA89550.028.5554945
CTCGATA9050.027.84501565
TATCAAC91850.027.5584642
TCAACGC94150.026.9326024
AACGCAG97000.026.7214286
GTACTAG13850.026.602651
ATCGTCA10550.026.47290410
AATGCGA12150.024.6976220
GCTCGAT10100.024.60845464
CGATAAT10400.024.56279867
ATGCGAC11250.024.52658821
TCGTCTA10200.023.68523254
GCTCGTC10600.023.44095239
ACGAGTC11250.023.30359826
Produced by FastQC (version 0.11.5)