FastQCFastQC Report
Sat 18 Jun 2016
SRR3137959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500915
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG40260.11499850753303065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34200.038.148261
GTATCAA65450.033.1703451
GCTCGAT10650.031.74766264
CTCGATA10700.031.59885865
TGGTCGT11250.030.3611351
TATGCCG12850.029.26526343
TCGTCTA12050.028.63177354
CTGGTCG12050.028.34545550
CGATAAT11750.028.18828467
CGACGAG7250.028.07490324
GTCAGCG12200.027.99654443
GCTCGTC12350.027.65571639
TCAGCGC12600.027.38158244
CTCGTAT12500.027.32384939
CAATGCG7650.027.05712919
TCGTATG14200.026.9681640
TGCGACG7700.026.43378422
CATTGCG12800.026.41391629
CAACGCA80250.026.1377985
CGTCATT13100.025.80827926
Produced by FastQC (version 0.11.5)