FastQCFastQC Report
Sat 18 Jun 2016
SRR3137958_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137958_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3533548
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG153620.4347471719642693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33700.042.7231861
GTATCAA65850.031.7504711
CAACGCA79850.026.1553945
ATCAACG78900.026.1534293
TCAACGC81850.025.3410364
AACGCAG84450.025.1445086
TATCAAC83600.024.8848842
CGTCGAG3350.021.61952210
ACGCAGA96600.021.594297
CGCAGAG99200.020.925828
CAATGCG3800.020.87893319
GTACATG97200.020.4468691
ATGCGAC4050.020.44185321
CGCGCGA850.00411031420.3039446
TACATGG98400.019.6718942
TGCGACG4150.019.11914422
AGAGTAC101000.019.05527711
GAGTACT59800.018.45605712
ACATGGG99000.018.4424883
CCGTCGA3950.018.3329439
Produced by FastQC (version 0.11.5)