FastQCFastQC Report
Sat 18 Jun 2016
SRR3137958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3533548
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31500.039.9893461
GTATCAA62400.033.737061
ATCAACG75750.027.0076353
CAACGCA78050.026.2555965
TATCAAC79600.026.1784842
TCAACGC78600.026.0718754
TATGCCG14750.025.72963343
AACGCAG83400.024.902266
TCGTATG15600.024.32735340
TCTCGTA15100.023.30471838
CTCGTAT15200.023.15172239
CGTACTA16650.022.3781429
GCTCGAT4800.022.28195864
ATGCCGT17050.021.85406944
TGCCGTC17500.021.09495545
ACGCAGA96250.020.9315787
TGCGACG3800.020.8804722
CGTATGC18600.020.40358541
CCGTACT18650.020.3480228
GATCTCG17250.020.2000736
Produced by FastQC (version 0.11.5)