FastQCFastQC Report
Sat 18 Jun 2016
SRR3137957_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137957_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729046
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106030.3885240483304422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.038.1208231
GTATCAA47050.032.938181
ATCAACG57150.026.3997753
CGACGAG5100.026.38049124
GCTCGAT4200.026.29787464
CAACGCA58250.026.2123055
CAATGCG4800.025.8693719
TGCGACG4850.025.60408822
TATCAAC59400.025.5096742
TCAACGC60600.025.016474
AACGCAG62950.024.642576
ATGCGAC5550.024.23974821
ATCGTCA5250.023.64634510
CTCGATA5050.021.87190465
CGATAAT5400.021.7334767
ACGCAGA69450.021.597627
GCGCGTA800.002898534821.56494330
CGCAGAG71450.021.0409438
GTACTAG8900.020.8953881
AATGCGA6700.020.5929120
Produced by FastQC (version 0.11.5)