FastQCFastQC Report
Sat 18 Jun 2016
SRR3137957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729046
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24300.037.642391
GTATCAA47700.034.0108151
TGCGACG3650.032.13607422
CGACGAG4050.028.9626724
ATCAACG57700.026.9066353
CAACGCA59200.026.3997125
TCAACGC62200.025.1264134
TATCAAC63950.025.087592
CAATGCG4850.024.89528819
TCGACGA2650.024.73605351
AACGCAG64800.024.5969736
ATCGTCA5700.024.20895210
GCTCGAT4150.024.1081964
ATGCGAC5500.023.83568621
CTCGTAT10350.023.66686239
TATGCCG11000.023.52205843
CGATAAT4600.023.25061667
CTCGATA4700.022.75508965
TCGTATG11800.021.92774640
CGACGAA3000.021.8501852
Produced by FastQC (version 0.11.5)