FastQCFastQC Report
Sat 18 Jun 2016
SRR3137956_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137956_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841754
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162470.571724364600173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.041.009091
GTATCAA56250.036.855371
TGCGACG6400.030.71818522
CAACGCA72450.028.536455
CAATGCG6800.028.4000119
ATCAACG71550.028.3551523
TATCAAC73550.027.9967162
ATGCGAC7550.027.86313221
TCAACGC75650.026.9587864
AACGCAG78100.026.7409956
CGACGAG7750.025.37220224
GTACTAG11150.024.090541
CGCACTA7550.023.77231258
ACGCAGA86050.023.6342247
GCGACGA8850.022.99738123
ATCGTCA8700.022.58692610
CGCAGAG90850.022.3112168
AATGCGA9050.022.1025220
GCATCGT8800.021.9369478
ACGAGTC9250.021.63264326
Produced by FastQC (version 0.11.5)