FastQCFastQC Report
Sat 18 Jun 2016
SRR3137956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841754
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG34010.11967960632764131TruSeq Adapter, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.040.822821
GTATCAA55100.035.5774841
TGCGACG4500.028.36559522
ATCAACG71300.026.4216143
TATCAAC73500.026.3818452
TATGCCG16400.026.0848443
CAACGCA73250.025.765345
CTCGTAT16050.025.57937239
ATCTCGT16300.025.18705437
CTCGATA6350.024.99206265
TCGTATG17250.024.59950440
TCAACGC77050.024.584194
GCTCGAT6600.024.5681264
AACGCAG78350.024.2198946
TCTCGTA16800.024.02672438
GATCTCG17800.022.87072636
CAATGCG5400.022.36026819
TGGTCGT7350.022.06116951
TGCCGTC19700.021.7156845
CGTACTA19350.021.57358729
Produced by FastQC (version 0.11.5)