FastQCFastQC Report
Sat 18 Jun 2016
SRR3137955_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137955_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911012
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210560.7233223360123558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39200.044.531341
GTATCAA85050.035.746231
CGACGAG4900.033.78598824
CAATGCG5650.031.12868719
ATCAACG106050.028.094493
CAACGCA109800.027.4291065
TATCAAC111950.027.099142
TGCGACG6150.026.91845322
TCAACGC114200.026.2443684
GCTCGAT6200.026.16853164
AACGCAG117450.026.0871166
ATCGTCA6500.025.46066310
TTCGCGT1251.2655073E-724.84083230
GCGCGAT3950.024.4447213
TGCGCGA4250.023.5289912
TCGATAA7200.023.49493466
CTCGATA6950.023.34660165
TGGTCGT7100.022.85060551
ACGCAGA132500.022.6084587
ACGTGCG4300.022.4488479
Produced by FastQC (version 0.11.5)