FastQCFastQC Report
Sat 18 Jun 2016
SRR3137955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911012
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG49840.17121193591781828TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG29720.10209507896223032TruSeq Adapter, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.042.9042551
CGACGAG5250.035.48568724
GTATCAA85000.035.2869071
CAATGCG6150.030.291119
TATGCCG21450.027.34216943
ATCAACG107700.027.2618313
TGCGACG6900.026.99951722
TATCAAC111600.026.682412
GCTCGAT7900.026.63967964
CAACGCA110750.026.4794455
TGGTCGT8150.026.24537851
CTCGATA8300.026.18717665
ATCGTCA7250.026.17194610
CTCGTAT21650.026.13303439
TCGTATG22550.026.00795640
TCTCGTA21650.025.6554338
TCAACGC115000.025.6508624
AACGCAG118350.025.1862936
GCTCGTC8650.024.72745539
TCAGCGC9050.024.39735244
Produced by FastQC (version 0.11.5)