FastQCFastQC Report
Sat 18 Jun 2016
SRR3137954_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137954_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2990894
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180980.605103357056452No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG37960.12691857351012772No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC30640.10244428588910205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38300.039.3924521
GTATCAA77300.035.693631
TCGATAA10750.030.18585866
GCTCGAT10700.030.00428664
TGGTCGT11250.029.14720551
CGACGAG7150.028.9439524
CTCGATA11200.028.66577165
CAATGCG7900.027.5017619
CGATAAT11800.027.50075167
ATCAACG97550.027.4440273
TCGTCTA11700.027.43705454
CAACGCA99100.027.2979555
TATCAAC103400.026.351232
GCGCGAT5650.026.24359713
ACGTGCG5700.026.0077239
AACGCAG108650.025.5371846
GCTCGTC12650.025.37145639
ATCGTCA8700.025.3628810
GTCGTCT12750.025.1754253
CTGGTCG13050.025.12648250
Produced by FastQC (version 0.11.5)