FastQCFastQC Report
Sat 18 Jun 2016
SRR3137954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2990894
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG43330.1448730713960441No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG39340.13153257855343586TruSeq Adapter, Index 11 (96% over 28bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC33180.11093672995432136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37450.038.984431
GTATCAA76650.035.347621
CGACGAG9250.033.566624
GCTCGAT9600.033.42314564
CAATGCG9800.032.73775519
CTCGATA9950.032.24745665
TGCGACG9750.032.2001522
CGATAAT10400.031.1833667
TCGATAA10450.031.03467466
TGGTCGT11000.029.7960951
TCGTCTA11250.029.13395554
GTCGTCT11550.028.67593653
TATGCCG18100.027.82932343
ATCGTCA11200.027.72056210
ATCAACG95850.027.0674173
CGCACTA11250.026.98814658
TATCAAC101600.026.4872262
CTCGTAT18000.026.45012339
CAACGCA98300.026.3226035
GCTCGTC12850.025.77443939
Produced by FastQC (version 0.11.5)