FastQCFastQC Report
Sat 18 Jun 2016
SRR3137953_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137953_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4660147
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149670.32117012617842317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.036.4726871
GTATCAA79650.032.820081
ATCAACG91700.027.6830833
CAACGCA95600.026.9610865
TCAACGC94400.026.9327074
TATCAAC99050.025.9034392
AACGCAG100950.025.6404346
CGACGAG4350.022.20296324
ACGCAGA115400.021.9286967
CGCAGAG119100.021.017688
GTACATG117200.019.7187121
ATGCGAC5250.019.70973621
AGAGTAC122400.019.10238811
TGCGACG4900.019.0060222
TACATGG116000.018.8822942
GTGTAGC25250.018.4143331
ACATGGG114750.018.2501813
CTCGATA4950.018.13127365
CAGAGTA132150.018.05680510
GAGTACT80700.018.0340112
Produced by FastQC (version 0.11.5)