FastQCFastQC Report
Sat 18 Jun 2016
SRR3137953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4660147
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38550.037.1579251
GTATCAA75900.033.8799131
ATCAACG90150.027.975573
CAACGCA91800.027.5103245
TCAACGC93450.026.987674
TATGCCG10250.026.5907543
TGCGACG4500.026.06604222
TATCAAC98050.026.0038812
AACGCAG98300.025.8310646
AATCTCG12900.024.8720336
GCGAATC15400.024.64279433
GCTCGAT5700.023.60545564
CTCGATA5750.023.40019465
CTCGTAT11950.023.38487839
CGACGAG5650.022.59237924
ACGCAGA113500.021.8842337
CGAATCT15350.021.57649434
TCTCGTA13200.021.170438
ATGCGAC6250.020.97549821
TCGTATG13150.020.72618540
Produced by FastQC (version 0.11.5)