FastQCFastQC Report
Sat 18 Jun 2016
SRR3137952_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137952_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5546673
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG535300.965083032657595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46850.039.3771931
GTATCAA96200.035.373191
ATCAACG114800.028.8244363
TCAACGC115250.028.795744
CAACGCA116900.028.3587635
AACGCAG122450.027.0734146
TATCAAC123500.026.9185142
CGCAGAG140000.023.1621178
ACGCAGA140600.023.1123457
CGCCGTA3100.021.1431853
AGAGTAC144800.020.84689511
GAGTACT92150.020.10537512
GTACTTT92600.019.71120514
CAGAGTA159500.019.48741510
GCAGAGT165000.019.046579
AGTACTT100200.018.38798513
GTGTAGC41800.017.8521521
TAGCCCT47000.017.7628064
GTACATG127750.017.3344551
ACTTTTT106650.017.11492516
Produced by FastQC (version 0.11.5)