FastQCFastQC Report
Sat 18 Jun 2016
SRR3137952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5546673
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG215450.3884310468635883TruSeq Adapter, Index 1 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG86740.156382032977246TruSeq Adapter, Index 1 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51700.042.515261
TATGCCG55200.035.81173743
CTCGTAT52150.035.45849239
GTATCAA111500.035.1561
TCTCGTA53100.034.75914438
ATGCCGT58050.034.0535344
TGCCGTC57450.033.98882745
CCGTCTT58550.033.40919547
GATCTCG55750.033.1069236
ATCTCGT56200.032.8418237
GCCGTCT59500.032.7018246
CGTATGC60450.032.4732541
TCGTATG60200.032.2642640
CGTCTTC62800.030.8735448
ATCAACG138250.027.7543893
GTATGCC72800.027.58045242
TCAACGC139050.027.5698934
CAACGCA141400.027.1849045
TATCAAC143950.026.799462
AACGCAG148600.025.8445156
Produced by FastQC (version 0.11.5)