FastQCFastQC Report
Sat 18 Jun 2016
SRR3137951_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137951_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5701602
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG408050.7156760503451486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64150.040.6012461
GTATCAA131700.033.913881
CGACGAG9200.030.00030524
ATCAACG155850.027.7844853
CAACGCA160400.027.1421495
GCTCGAT10550.027.14031464
TATCAAC164800.026.6443332
TGCGACG10300.026.46189922
TCAACGC166300.026.1181094
AACGCAG168900.026.0417256
CAATGCG10700.025.47423219
TGGTCGT11400.024.81408351
CTCGATA12400.023.09115265
ACGCAGA192450.022.3710257
CGCAGAG194200.022.1339058
CGCACTA12500.021.802558
GCTCGTC13350.021.44852839
GTGGTAT24850.021.268151
GTACTAG17750.021.2125471
ATGCGAC13900.021.0982621
Produced by FastQC (version 0.11.5)