FastQCFastQC Report
Sat 18 Jun 2016
SRR3137951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5701602
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG136990.2402658059962796TruSeq Adapter, Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG66160.11603756277621623TruSeq Adapter, Index 8 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70750.042.4294931
GTATCAA150100.035.8837051
TATGCCG43000.031.29061543
AATCTCG43300.030.19684836
CTCGTAT43800.029.616139
TCTCGTA43750.029.3345238
TCGTATG45500.028.73728640
ATCAACG185200.028.5800673
CGACGAG9700.028.09712624
TATCAAC192550.027.8831042
CAACGCA190550.027.868915
TGCCGTC47900.027.51325645
TGCGACG10050.027.46189122
ATGCCGT48700.027.34489444
TCAACGC195600.027.2020744
AACGCAG201050.026.7223636
GCTCGAT11750.026.4252364
CCGTCTT50050.026.26243647
CGTCTTC50400.026.0800648
ATCTCGT50500.025.68683637
Produced by FastQC (version 0.11.5)