FastQCFastQC Report
Sat 18 Jun 2016
SRR3137950_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137950_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558161
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43030.16820677041046284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.039.2319871
GTATCAA48850.031.53331
ATCAACG54300.027.1311723
TCAACGC56150.026.2961434
CAACGCA56950.025.8661735
AACGCAG60650.024.6863576
TATCAAC60800.024.637492
GTGGTAT12250.023.9652521
CGCAGAG69000.022.1489288
ACGCAGA72350.020.5988647
AGAGTAC72400.020.15618511
GAGTACT55550.019.93551612
ACCGTGC2950.019.8803948
GTACATG53200.019.6062471
TGGTATC13650.019.217252
TACATGG52800.019.022582
GCAGAGT80150.018.8094449
CAGAGTA78800.018.73803710
GATAACG5000.018.62909762
ACATGGG50250.018.6068863
Produced by FastQC (version 0.11.5)