FastQCFastQC Report
Sat 18 Jun 2016
SRR3137950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558161
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24600.043.200741
GTATCAA52650.034.6027871
TCAACGC64400.027.9143474
ATCAACG64600.027.8279233
CAACGCA65750.027.4986345
TGCGACG1800.026.83262622
TATCAAC67800.026.819862
AACGCAG70450.025.8599996
TATGCCG4750.023.96825843
AATCTCG4300.023.26728436
CGCAGAG77700.023.2657958
TCTCGTA4400.022.73892638
ACGCAGA81250.022.2505657
AATGCGA2251.0913936E-1121.466120
AGAGTAC82150.021.41841111
CGTATTC1808.596544E-921.08277729
CTCGTAT4750.021.06342539
GAGTACT66550.021.047612
TCGTATG5950.020.87421640
GTCGTAG1507.330218E-720.7026921
Produced by FastQC (version 0.11.5)