FastQCFastQC Report
Sat 18 Jun 2016
SRR3137947_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137947_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464207
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG709454.845284853849217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.047.801371
GTATCAA34100.035.268521
ATCAACG43500.026.9683483
CTCGTCG2050.026.9250569
CGTCGCA1800.026.83248111
CAACGCA44750.026.6721925
TCAACGC44350.026.6025334
TATCAAC45450.026.2078742
CCCGCGA1607.8216544E-1125.87683121
CGTATCA1208.529787E-825.87241256
AACGCAG46600.025.8354026
CTCGCGC1501.0440999E-925.2974760
TATCGGC1251.2558303E-724.858742
TCGCGCT1551.5279511E-924.48142461
CGCATTG2001.8189894E-1224.15170914
CTCGATA2650.023.4316265
GCTCGAT2650.023.4316264
ACGCAGA51600.022.8639957
CGCAGAG52050.022.5337728
CGCGATC1854.838512E-1022.37996323
Produced by FastQC (version 0.11.5)