Basic Statistics
Measure | Value |
---|---|
Filename | SRR3137947_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1464207 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 23971 | 1.6371319082616052 | RNA PCR Primer, Index 46 (96% over 28bp) |
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG | 13323 | 0.9099123279700206 | RNA PCR Primer, Index 46 (96% over 28bp) |
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG | 8434 | 0.5760114519326844 | RNA PCR Primer, Index 46 (96% over 28bp) |
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG | 1859 | 0.1269629225922291 | TruSeq Adapter, Index 4 (96% over 27bp) |
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG | 1642 | 0.11214261371513727 | TruSeq Adapter, Index 4 (96% over 27bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 1940 | 0.0 | 47.840446 | 1 |
GTATCAA | 4100 | 0.0 | 37.363174 | 1 |
TCTCGTA | 6710 | 0.0 | 32.59731 | 38 |
CTCGTAT | 6795 | 0.0 | 32.54495 | 39 |
TCGTATG | 7085 | 0.0 | 32.478878 | 40 |
AATCTCG | 6805 | 0.0 | 32.39573 | 36 |
TATGCCG | 7145 | 0.0 | 32.35099 | 43 |
ATGCCGT | 7160 | 0.0 | 32.186848 | 44 |
ATCTCGT | 6860 | 0.0 | 32.03541 | 37 |
CGTATGC | 7270 | 0.0 | 31.699839 | 41 |
TGCCGTC | 7210 | 0.0 | 31.580841 | 45 |
CGTCTTC | 6985 | 0.0 | 31.511513 | 48 |
CGAATCT | 7120 | 0.0 | 31.398582 | 34 |
CCGTCTT | 7155 | 0.0 | 31.341425 | 47 |
GCGAATC | 7375 | 0.0 | 31.2953 | 33 |
GCCGTCT | 7185 | 0.0 | 30.874449 | 46 |
GAATCTC | 7200 | 0.0 | 30.858044 | 35 |
GTATGCC | 7510 | 0.0 | 30.77867 | 42 |
GCTTGAA | 7565 | 0.0 | 30.14446 | 56 |
TGCTTGA | 7470 | 0.0 | 29.973614 | 55 |