FastQCFastQC Report
Sat 18 Jun 2016
SRR3137947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464207
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG239711.6371319082616052RNA PCR Primer, Index 46 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG133230.9099123279700206RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG84340.5760114519326844RNA PCR Primer, Index 46 (96% over 28bp)
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG18590.1269629225922291TruSeq Adapter, Index 4 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG16420.11214261371513727TruSeq Adapter, Index 4 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.047.8404461
GTATCAA41000.037.3631741
TCTCGTA67100.032.5973138
CTCGTAT67950.032.5449539
TCGTATG70850.032.47887840
AATCTCG68050.032.3957336
TATGCCG71450.032.3509943
ATGCCGT71600.032.18684844
ATCTCGT68600.032.0354137
CGTATGC72700.031.69983941
TGCCGTC72100.031.58084145
CGTCTTC69850.031.51151348
CGAATCT71200.031.39858234
CCGTCTT71550.031.34142547
GCGAATC73750.031.295333
GCCGTCT71850.030.87444946
GAATCTC72000.030.85804435
GTATGCC75100.030.7786742
GCTTGAA75650.030.1444656
TGCTTGA74700.029.97361455