FastQCFastQC Report
Sat 18 Jun 2016
SRR3137945_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137945_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6288741
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290620.46212747511783364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63500.044.8803861
GTATCAA141000.035.3037831
ATCAACG173800.027.6357773
TCAACGC175550.027.3164124
CAACGCA176250.027.2646925
TATCAAC181650.026.890582
AACGCAG185750.025.907416
CAATGCG9100.023.88732719
CGCACTA8400.023.81920658
ATGCGAC10500.023.00261121
CGACGAG8600.022.86832824
TGGTCGT10400.022.5555951
ACGCAGA210650.022.353727
CGCAGAG212950.022.1446848
CTCGATA10550.021.9079165
AATGCGA9950.021.50009720
TGCGACG9400.021.28931222
GCTCGTC12350.020.39194739
GCTCGAT11450.020.1858964
AGAGTAC220500.020.16662811
Produced by FastQC (version 0.11.5)