FastQCFastQC Report
Sat 18 Jun 2016
SRR3137945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6288741
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG100450.15972990460252698TruSeq Adapter, Index 3 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72000.047.2041741
GTATCAA163950.035.293791
CTCGTAT28650.030.70648239
AATCTCG28350.030.6663436
TATGCCG31150.029.79238543
TCTCGTA30000.028.97969238
ATCAACG206050.027.8314023
TCAACGC211150.027.1755164
CAACGCA211700.027.1212145
CCGTCTT34100.027.11408247
TCGTATG34800.026.76695640
TATCAAC215550.026.7168312
ATCTCGT32950.026.28044937
AACGCAG222800.025.8939176
ATGCCGT36250.025.88641544
TGCCGTC35800.025.82633845
GCCGTCT36500.025.614846
CTCGATA9250.025.3616965
TGGTCGT9950.025.31149151
CGACGAG8800.024.69860324
Produced by FastQC (version 0.11.5)