FastQCFastQC Report
Sat 18 Jun 2016
SRR3137944_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137944_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5585667
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205040.36708239141359483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50650.039.5563771
GTATCAA108500.034.925681
CGACGAG4800.033.06548724
TGCGACG5250.030.8887822
ATCAACG125400.029.0547333
TCAACGC128550.028.1782054
CAACGCA129650.028.1778555
TATCAAC134400.027.482532
AACGCAG136050.027.0551746
ATGCGAC6900.026.50263221
ACGCAGA151950.024.0881257
CGCAGAG155550.023.8635188
CAATGCG6800.023.8481719
GCGACGA6700.023.17394323
GCTCGAT5900.022.80326564
AATGCGA7300.022.68718320
ATCGTCA7300.022.21115310
GTCGTCT6750.021.97602553
AGAGTAC163900.021.34355511
GAGTACT116450.021.0644212
Produced by FastQC (version 0.11.5)