FastQCFastQC Report
Sat 18 Jun 2016
SRR3137944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5585667
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG72860.13044100194300878TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57700.043.116731
GTATCAA129100.035.8951761
CGACGAG4000.032.77420424
TATGCCG21000.029.73525843
ATCAACG155200.029.2139283
CTCGTAT20450.028.8479739
TCAACGC159450.028.4568964
TCTCGTA20150.028.421438
CAACGCA161100.028.1876145
TATCAAC163400.028.0012742
TGCGACG4750.027.59957922
ATGCCGT23200.027.21294644
AACGCAG169050.027.0253166
TCGTATG23750.026.58275240
TGCCGTC23900.026.27156645
GCCGTCT25150.024.9658246
CCGTCTT25550.024.84502247
CAATGCG5800.023.7927419
ACGCAGA191550.023.6853287
CGCAGAG193250.023.3868648
Produced by FastQC (version 0.11.5)