FastQCFastQC Report
Sat 18 Jun 2016
SRR3137943_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137943_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5297247
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG255920.4831188728786858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53000.042.3645361
GTATCAA117800.034.543391
ATCAACG139100.028.6501923
TCAACGC142150.028.1283114
CAACGCA142500.028.106855
AACGCAG149800.026.8750786
TATCAAC150650.026.6265412
CGCAGAG169350.023.4873928
ACGCAGA170850.023.3013747
AGAGTAC172150.021.38259311
GAGTACT111050.021.37417212
GCAGAGT187000.020.6433039
CAGAGTA184250.020.40882110
GTACTTT118650.019.94823514
TATACCG3000.019.5489065
AGTACTT125350.018.96403113
CGTCGAG3300.018.81730710
GTGTAGC27550.018.055351
GTACATG138100.018.0346031
GCTCGAT4700.017.6158764
Produced by FastQC (version 0.11.5)