FastQCFastQC Report
Sat 18 Jun 2016
SRR3137943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5297247
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG99280.18741810604640485RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60950.046.929451
GTATCAA148450.037.9783861
TATGCCG26700.031.52752943
CTCGTAT26800.030.50878539
ATCAACG186500.029.6194633
TCTCGTA28000.029.07805638
TCAACGC191150.028.8989244
CAACGCA191650.028.8778115
TATCAAC192700.028.7560022
AACGCAG198600.027.9888526
TATCTCG29250.027.59950836
ATGCCGT30400.027.5770744
TGCCGTC30400.027.46358545
GCCGTCT31200.026.98079946
CCGTCTT31100.026.95662347
ATCTCGT31650.025.50665737
TCGTATG33400.025.30643740
ACGCAGA225100.024.3849817
CGCAGAG224550.024.3057428
CGTCGCG1151.69956E-624.00002758
Produced by FastQC (version 0.11.5)