FastQCFastQC Report
Sat 18 Jun 2016
SRR3137942_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137942_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5509112
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333650.605632994936389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57100.042.6437571
GTATCAA115550.035.68921
ATCAACG142000.028.7673873
CAACGCA146450.028.0786765
TCAACGC146150.028.0424044
TATCAAC149050.027.5608442
AACGCAG150950.027.44736
ACGCAGA175100.023.6026297
CGACGAG2500.023.46118424
CGCAGAG179200.023.351388
AGAGTAC183250.021.33002711
GCAGAGT195650.020.8414219
GAGTACT123900.020.60542912
CAGAGTA196400.020.51611110
TCGTCCG3000.019.54885710
AGTACTT133050.019.44820213
GTACATG152250.019.2825321
GTACTTT130550.019.1867214
TACATGG151250.018.6924442
GTGTAGC30900.018.331071
Produced by FastQC (version 0.11.5)