FastQCFastQC Report
Sat 18 Jun 2016
SRR3137942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5509112
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG122870.22303049928917765TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG56740.10299300504328102TruSeq Adapter, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.046.374141
GTATCAA149750.035.4165341
TATGCCG34400.032.19251643
CTCGTAT34000.031.150739
ATGCCGT36000.030.8575744
TCTCGTA34350.030.53199638
TGCCGTC36800.030.28050245
TCGTATG36600.030.25744840
GCCGTCT37950.029.27200346
CCGTCTT38300.028.91469247
GATCTCG36900.028.8895336
ATCAACG187900.028.1156043
CGTACTA39300.027.82755529
CAACGCA192150.027.5840325
TCAACGC192000.027.5336914
TATCAAC193650.027.3342342
ATCTCGT39100.027.26403237
CGTATGC40950.027.12753541
CGTCTTC41350.027.11565848
AACGCAG200000.026.7601596
Produced by FastQC (version 0.11.5)