FastQCFastQC Report
Sat 18 Jun 2016
SRR3137939_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137939_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4199140
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG539991.2859537905380625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39050.039.8956831
GTATCAA81150.034.5225371
CAACGCA98200.027.4369345
ATCAACG99150.027.1428073
TATCAAC102700.026.9245072
TCAACGC100350.026.8837954
AACGCAG103800.026.1561266
GCTCGAT6550.023.70102964
CGCAGAG118600.022.5721558
CGACGAG7000.022.17897824
ACGCAGA120250.022.1476787
TAACGCC6700.021.6259754
AATGCGA7400.021.44659420
AGAGTAC121300.020.53443311
ATCGTCA7900.020.52447110
CTCGATA7750.020.03119365
TGGTCGT7800.019.90278851
CAGAGTA128400.019.82862310
TCGATAA8250.019.653566
CAATGCG8050.019.28652819
Produced by FastQC (version 0.11.5)