FastQCFastQC Report
Sat 18 Jun 2016
SRR3137939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4199140
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG195710.46607162418971504TruSeq Adapter, Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG99330.23654843610834597TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG52220.12435879727753778RNA PCR Primer, Index 3 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45300.042.0468831
GTATCAA94800.034.2874761
AATCTCG51900.031.70744136
CTCGTAT52350.031.50078639
TATGCCG56250.030.85003543
ATCTCGT53650.030.67318337
TCTCGTA53900.030.46690638
TCGTATG58200.030.05350740
CCGTCTT59050.028.9198147
CGTATGC61000.028.61744541
ATGCCGT60800.028.37112844
TGCCGTC60800.028.31438645
CGTCTTC60900.027.814748
ATCAACG114950.027.4972843
CGACGAG6200.027.26524424
CAACGCA116250.027.2197935
TCAACGC117650.026.8369054
GCCGTCT63850.026.74573346
GAATCTC62400.026.59320435
TATCAAC119600.026.5718252
Produced by FastQC (version 0.11.5)