FastQCFastQC Report
Sat 18 Jun 2016
SRR3137938_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137938_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7074631
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG606100.8567231280331088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54600.039.41011
GTATCAA116100.034.3904381
ATCAACG135750.028.1113723
TCAACGC137950.027.609134
CAACGCA139150.027.4944155
AACGCAG146000.026.4879826
TATCAAC153950.025.1131742
CGTACGA4400.022.73743460
CGCAGAG168000.022.6086258
ACGCAGA172000.022.1430157
ACGTGCG3450.021.9988379
ACCGTCG4750.021.79001623
GCTCGAT8100.020.86926764
AGAGTAC176600.020.43395611
TGCGACG5750.019.80049122
CAGAGTA188550.019.54087310
CGACGAG5500.019.445824
GCAGAGT193950.019.2634839
GATAACG15650.019.17790262
GAGTACT103200.018.78759612
Produced by FastQC (version 0.11.5)