FastQCFastQC Report
Sat 18 Jun 2016
SRR3137938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7074631
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG242190.34233587589232567RNA PCR Primer, Index 33 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG106620.15070750686502235RNA PCR Primer, Index 33 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63900.042.281361
GTATCAA133350.034.363161
CTCGTAT58050.032.9247139
TATGCCG63700.032.17053643
AATCTCG59750.031.69901336
TCTCGTA60300.031.69617338
TGCCGTC67550.030.08183345
ATGCCGT68250.029.97549844
TCGTATG69300.029.67067140
ATCTCGT64350.029.64748837
CCGTCTT68550.029.54234947
GCCGTCT71050.028.5999746
CGTCTTC73100.027.75072748
ATCAACG163700.027.697133
CAACGCA165850.027.3172685
TCAACGC166550.027.2438934
CGTATGC75800.027.08084141
AACGCAG174150.026.2725356
GGCAATC73050.026.25825733
TATCAAC178000.025.5883312
Produced by FastQC (version 0.11.5)