FastQCFastQC Report
Sat 18 Jun 2016
SRR3137937_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137937_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4304539
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110300.2562411445220963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35400.039.4261051
GTATCAA74300.034.221161
ATCAACG87300.027.9823743
TCAACGC88200.027.7311254
CAACGCA89200.027.4969485
GCTCGAT3650.027.40847664
AACGCAG93200.026.6129346
TATCAAC95100.026.027732
CGACGAG3000.024.15230224
CGCAGAG109700.022.5471828
ACGCAGA109700.022.4528437
GCTCGTC4750.021.0617439
GTGTAGC28800.020.7519721
AGAGTAC115800.020.22861911
CTCGATA4950.020.2102965
GAGTACT72450.020.0955212
TAGCCCT32300.019.972344
CGCACTA3650.019.84751758
CAGAGTA122500.019.48766510
TGCGACG3900.019.46339222
Produced by FastQC (version 0.11.5)