FastQCFastQC Report
Sat 18 Jun 2016
SRR3137937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4304539
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40450.044.5267941
GTATCAA85700.034.463741
TATGCCG12050.027.7714643
ATCAACG106300.027.233173
TCAACGC107850.026.8424024
CAACGCA108600.026.7847375
TGCCGTC12650.026.45423345
TATCAAC112900.026.038452
CTCGTAT11500.025.79969639
AACGCAG114100.025.7053156
GCGCGAT951.0516433E-525.42104713
TCTCGTA11900.024.35265438
ATGCCGT13950.024.23627344
CGTGCGC9000.023.38441710
CGACGAG3400.022.32300824
CCGTCTT14750.022.22007847
TCGCCCG5950.022.03309822
TCGTATG14600.021.97576740
ACGCAGA134200.021.6215957
CGCAGAG134400.021.4605668
Produced by FastQC (version 0.11.5)