FastQCFastQC Report
Sat 18 Jun 2016
SRR3137936_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137936_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4147636
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86230.20790156127490456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.038.1414221
GTATCAA77850.033.5439571
ATCAACG90800.028.1572973
TCAACGC92600.027.7932514
CAACGCA94750.027.2340955
TATCAAC97000.026.51392
AACGCAG99550.026.0942176
ACGCAGA111600.023.02947
CGCAGAG115150.022.7688038
ATCGACG1253.4880177E-622.093732
TAGCCCT28300.021.4554564
AGAGTAC117250.020.8021211
GAGTACT89100.020.25048612
CCGCTCG1206.395455E-520.1236769
CAGAGTA127250.019.73622710
GCAGAGT130100.019.7281429
GTGTAGC26600.019.2190191
GTACTTT93700.018.99951714
CTCGATA3850.018.81694665
GTGGTAT20100.018.3881841
Produced by FastQC (version 0.11.5)