FastQCFastQC Report
Sat 18 Jun 2016
SRR3137936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4147636
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44800.042.9747351
GTATCAA95450.035.3163381
TATGCCG8550.030.2632543
ATCAACG117150.028.4218643
TCAACGC118700.028.0216584
CTCGTAT9200.027.7500839
CAACGCA120400.027.7119755
TATCAAC120400.027.6253382
AACGCAG125050.026.9022396
TCTCGTA9850.025.9188638
TGCCGTC10200.024.69065545
ATGCCGT10750.023.74890944
ACGCAGA141100.023.7184377
CGTATGC10950.023.63020741
TCGTATG11050.023.41635940
CGCAGAG143350.023.3212458
CCGTCTT11350.022.1889647
GCCGTCT11550.021.80473346
AGAGTAC150650.021.48072211
ATCTCGT12100.021.09872837
Produced by FastQC (version 0.11.5)