FastQCFastQC Report
Sat 18 Jun 2016
SRR3137935_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137935_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6258859
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG359570.5744976840027871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58850.041.679621
GTATCAA123050.034.4207841
ATCAACG143500.028.6628173
CAACGCA146600.028.1674275
TCAACGC146650.028.1347474
AACGCAG152950.027.1335496
TATCAAC154600.026.7967112
TGCGACG4650.025.96893122
CGCAGAG175050.023.8064848
ACGCAGA175900.023.377657
CGTACGA3150.022.99829960
CGACGAG5400.022.36195824
GAGTACT118650.021.98207912
AGAGTAC181900.021.56379111
GCAGAGT197650.020.7178339
CAGAGTA196400.020.42829510
GCTCGAT6000.020.1235164
GTACTTT129850.019.87413414
AGTACTT135900.019.57311813
GGTCGTC10350.019.33190352
Produced by FastQC (version 0.11.5)