FastQCFastQC Report
Sat 18 Jun 2016
SRR3137935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6258859
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG139800.22336339578827388TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65850.043.1263851
GTATCAA149700.036.3964731
TATGCCG37950.035.5451843
CTCGTAT36600.035.15956539
TCTCGTA36050.034.9303838
GATCTCG38450.032.65982436
ATCTCGT39150.032.1639937
ATGCCGT42250.031.9275644
CCGTCTT42650.031.54673247
TGCCGTC43250.031.18885445
TCGTATG43800.030.79770740
ATCAACG183000.029.5474763
TCAACGC185600.029.2086244
CAACGCA186100.029.0930635
CGTCTTC47250.028.47551548
TATCAAC191250.028.3087372
CGTATGC47950.028.2041641
GCCGTCT48050.028.14501446
AACGCAG196850.027.662286
GTATGCC54500.025.06769442
Produced by FastQC (version 0.11.5)