FastQCFastQC Report
Sat 18 Jun 2016
SRR3137934_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137934_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5843610
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG248560.4253535057952191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54800.041.784821
GTATCAA118650.034.9882551
ATCAACG140600.028.8841933
TCAACGC141350.028.6286354
CAACGCA142850.028.2548475
TATCAAC148700.027.4627232
AACGCAG152850.026.6771456
CGCAGAG169450.023.8398028
ACGCAGA172100.023.4526717
AGAGTAC177700.021.51143511
GAGTACT120950.020.65176412
CAGAGTA189250.020.58035310
GTACTTT119300.020.50448414
GCAGAGT195850.020.2035279
GTACATG158150.019.4141371
AGTACTT128100.019.3650713
ACATGGG153700.018.520353
TACATGG164000.018.1440092
GTGGTAT25100.017.3373361
TACCGAC2802.564775E-1017.2487377
Produced by FastQC (version 0.11.5)