FastQCFastQC Report
Sat 18 Jun 2016
SRR3137934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5843610
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG99910.17097307999678282RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69150.047.1533551
GTATCAA154100.036.429891
TCTCGTA26600.031.12688638
TATGCCG26950.031.10720443
CTCGTAT27450.030.41464639
TATCTCG28200.029.11614836
ATCAACG193600.028.6233563
TCAACGC195550.028.373464
CCGTCTT30100.028.19613347
TATCAAC197650.028.0363642
CAACGCA198450.027.9938475
ATCTCGT29950.027.87562637
ATGCCGT30550.027.44154544
AACGCAG210850.026.576656
TGCCGTC31550.026.57176645
TCGTATG31900.026.28022640
CGTATGC33750.024.941941
GCCGTCT36050.023.63769746
CGCAGAG232950.023.5634868
ACGCAGA235300.023.417737
Produced by FastQC (version 0.11.5)