FastQCFastQC Report
Sat 18 Jun 2016
SRR3137933_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137933_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5828897
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG373030.639966703820637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65800.044.619181
GTATCAA142950.036.4614261
ATCAACG180550.028.1488323
CAACGCA181700.028.0807515
TCAACGC186350.027.2328854
CGACGAG8500.027.19497124
TATCAAC192550.026.835662
AACGCAG192550.026.5340376
ATGCGAC9650.026.09971221
TGCGACG9500.024.33276222
CGCAGAG221400.022.8738988
CAATGCG10550.022.56546619
ACGCAGA224800.022.5432877
AGAGTAC225350.021.44839111
GTACTAG14950.021.2556881
GAGTACT125350.020.72489212
CAGAGTA239200.020.59574110
GTACATG220700.020.5803131
GTGGTAT25600.020.3734951
CTCGATA10200.020.29284165
Produced by FastQC (version 0.11.5)