FastQCFastQC Report
Sat 18 Jun 2016
SRR3137933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5828897
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98110.16831657859111251No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG92750.1591210138041554RNA PCR Primer, Index 26 (100% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58290.10000176705815869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84800.048.3777541
GTATCAA192050.037.207071
CGACGAG8650.030.7104324
CTCGTAT29700.029.96941439
TATGCCG30700.029.4429743
TCTCGTA30750.028.94606838
ATCAACG250000.028.2512423
CAACGCA253200.027.9900635
ATCTCGT33300.027.76526637
TCAACGC255750.027.6165474
TATCAAC259150.027.4800932
AACGCAG264950.026.931086
CTCGATA11450.026.51480765
GCTCGAT11700.026.24311864
TGCGACG10350.025.99953322
ATGCCGT34750.025.91220744
TCGTATG33950.025.81124540
TGGTCGT12700.025.2635451
CGCAGAG297050.023.6006538
ACGCAGA302150.023.4086347
Produced by FastQC (version 0.11.5)